We are keen to develop new imaging technology for probing immunological recognition, often in collaboration with others.We have reported several uses for fluorescence lifetime imaging (FLIM) including imaging receptor phosphorylation [J Cell Biol. 2006; 174:153-61] and local refractive index [Biophys J. 2002; 83:3589-95, J Microsc. 2005; 217:36-43]. We also developed a method to image linear dichroism to probe lipid order and membrane ruffling [Biophys J. 2005; 88:609-22].

We used optical tweezers to obtain high-resolution images of immune synapses [Sci Signal. 2010; 3:ra36; Biophys J. 2008; 95 :L66-8.]. We also employed super-resolution microscope to visualise changes in actin structures at the NK cell immune synapse [Blood 2012; 120:3729-40; PLoS Biol. 2011; 9:e1001152]. We are currently applying a variety of different super-resolution imaging technologies to image morphological changes and signal integration during NK cell surveillance.

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